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The digestion of dietary protein is an important driving force for animal nutrition, as protein and amino acids are one of the most expensive nutrients. This is why different kinds of proteases are so widely used in the animal feed industry today.

Neutral protease, as an efficient enzyme preparation, has a wide range of applications across several industries such as the feed industry, leather and textile industry, beer making and baking industry, as well as soy protein isolate production, and HAP/HVP production.

Acidic Protease, as an important class of enzymes, plays an indispensable role in both biological organisms and industrial production. the wide range of applications and potential value of Acidic Protease in the food industry, feed industry, medical field, and environmental protection will be elaborated below.

Acidic Protease, as an important class of enzymes, plays an indispensable role in both biological organisms and industrial production. This paper aims to delve into the basic characteristics, mechanism of action, application fields, and future development trends of Acidic Protease, providing valuable references for researchers and practitioners in related fields.

With the rapid development of animal husbandry and the continuous progress of science and technology, the application prospects of alkaline protease in animal husbandry will be even broader. The future development trends mainly include the following aspects.

This article summarizes the current application status, mechanism of action, influencing factors, and future development trends of alkaline protease in animal husbandry, aiming to provide scientific basis and technical support for animal husbandry production.

With the rapid development of animal husbandry, the improvement of feed utilization and animal production performance has become a focus of industry attention. Alkaline protease, as an important feed additive, has shown broad application prospects in animal husbandry.

Protease is the general term for a class of enzymes that hydrolyze protein peptide bonds. According to the way it hydrolyzes peptides, it can be divided into two categories and multiple sub-categories.

​The testing methods for Bacillus licheniformis detection mainly include pure cultivation, ELISA, PCR, fluorescence quantitative PCR, and gene sequencing.

There are several advantages of using Bacillus subtilis and Bacillus licheniformis in combination, which can be mainly reflected in the following aspects.