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With the rapid development of animal husbandry and the continuous progress of science and technology, the application prospects of alkaline protease in animal husbandry will be even broader. The future development trends mainly include the following aspects.

This article summarizes the current application status, mechanism of action, influencing factors, and future development trends of alkaline protease in animal husbandry, aiming to provide scientific basis and technical support for animal husbandry production.

With the rapid development of animal husbandry, the improvement of feed utilization and animal production performance has become a focus of industry attention. Alkaline protease, as an important feed additive, has shown broad application prospects in animal husbandry.

Protease is the general term for a class of enzymes that hydrolyze protein peptide bonds. According to the way it hydrolyzes peptides, it can be divided into two categories and multiple sub-categories.

​The testing methods for Bacillus licheniformis detection mainly include pure cultivation, ELISA, PCR, fluorescence quantitative PCR, and gene sequencing.

There are several advantages of using Bacillus subtilis and Bacillus licheniformis in combination, which can be mainly reflected in the following aspects.

The testing methods for Bacillus subtilis mainly include traditional cultivation methods, molecular biology methods, and immunological methods. ‌

Bacillus licheniformis and Bacillus subtilis are two common microorganisms that can naturally coexist and grow together, forming a mutually beneficial relationship. In aquaculture, utilizing this relationship and combining the two can achieve various positive effects.

Based on experience and practice, the recommended mixing ratio for Bacillus subtilis and Bacillus licheniformis is 1:1 or 1:2. This ratio helps to fully leverage the synergistic effect of the two bacteria and improve application effectiveness.

Bacillus licheniformis and Bacillus subtilis are two common microorganisms that can naturally coexist and grow together, forming a mutually beneficial relationship. By utilizing this relationship, the two can be combined in aquaculture to improve breeding efficiency and prevent diseases.