High Temperature Resistant Phytase

The thermostable phytase is a new generation of phytase developed by Starzyme. Compared with other phytases, it has the advantages of high thermostability, convenience for use, and high biological efficiency.  The thermostable phytase is suitable for high temperature pelleting of compound feeds of livestock and poultry.  The thermostable phytase will widen the field of application of phytase and make new contributions to the development of feed industry

Features:

1. Appearance: light brownish pellets with 30-60 mesh

2. Activity: ≥5,000 U/g, ≥10,000 U/g

3. Specialized for pelleting at high temperature

Definition of the unit of the enzyme activity: One unit (U) is defined as the amount of enzyme needed to hydrolyze sodium phytate (5.0 mmol/L) to release 1 μmol of inorganic phosphorus (based on dipotassium phosphate measured by absorbance value in the chromogenic reaction with ammonium nitrate, ammonium molybdate and ammonium alum) using triton and bovine serum albumin as surfactants in acetic acid-sodium acetate buffer (0.25 mol/L, pH 5.5) at 37°C in 1 min (total reaction time is 30 min). (GB/T18634-2009)

Mechanisms:

1. The thermostability of the thermostable phytase has been enhanced by    site-directed mutagenesis:

The structure of the phytase protein was modified by sequence changes of the gene and amino acids using site-directed mutagenesis.  The mutated gene was obtained by large screening and expressed in a bioreactor, which resulted in the mutated phytase with high thermostability.

2. The thermostability of the thermostable phytase has been increased by molecular fixation technique:

The enzymatic conformation was firmly fixed by connecting the enzyme with a vector in multiple sites, which prevented the transition from a folded state to an extended state and thus increased the enzymatic thermostability. Meanwhile, the enzyme became more resistant to pepsin digestion and autolysis.

Applications:

The amount of the thermostable phytase (5,000 U/g) to be added in compound feeds varies at different temperatures.

70-80 ⁰C: 80-100 g/T, 80-85⁰C: 90-110 g/T, 85-90 ⁰C:100-120 g/T.

Specification: 20 kg/bag

Storage:

Store in dry and cool (below 25°C) area with ventilation. Keep away from light.

Shelf life: 12 months (at 25°C and relative humidity≤60%).

Analysis on the current pain points of the industry

1. False mark of enzyme activity

The product is marked with 100,000 enzyme activities, and the actual enzyme activity is 60,000, resulting in incomplete hydrolysis and indirectly increasing the cost of enzymes.

2. Product is unstable

Lack of enzyme protection technology and quality control, unstable enzyme activity, and differences in product specifications for each batch, resulting in product quality fluctuations.

3. Long production cycle

No enzymatic hydrolysis experience and enzyme compounding technology increase labor cost and R&D cycle, which indirectly affects the production schedule.

For us, our major is to solve these pain points

1. The enzyme activity can be customized, providing different enzyme activity products and solutions

2. Stable enzyme activity, unique enzyme activity protection technology to reduce fluctuations in production quality

3. Enzyme activity is not false, strict quality control links, third-party testing to reduce enzyme costs

Quality control strength
6 key control point inspections, escorting the whole process of quality, using extraction, membrane separation, vacuum freeze-drying, spray drying and enzyme activity protection technologies to produce products that fully meet the requirements of global customers.

Production capacity

  Eight automatic primary fermentation cylinders (60 m³),

　　2 pure culture tanks (10 m³),      2 pure culture tanks (5 m³), 

       4 pure culture tank (4 m³),         2 pure culture tanks (1 m³),

　　4 pure culture tanks (0.3 m³),     1 large air compressors,

　　1 spray/drying tower (1 ton/h),

　　4 air drying and fluidized bed drying equipment

　　1 production line of ultra concentrate liquid enzyme.

  The annual production capacity of the Starzyme is more than 20,000 tons of liquid, powd

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